Background: EF-1 (elongation factor-1) is a multi-protein complex that is comprised of α, β, γ and δ subunits, all of which work together to ensure the delivery of aminoacyl-tRNAs to the ribosome, thereby elongating mRNA. EF-1 ε1 (eukaryotic translation elongation factor 1 epsilon-1), also known as multisynthetase complex auxiliary component p18, is a 174 amino acid protein that shares sequence similarity with the amino-terminal ends of the β and γ subunits of EF-1. By specifically interacting with MetRS, EF-1 ε1 binds to a macromolecular tRNA synthtase complex that catalyzes the ligation of specific amino acids to their cognate tRNAs. Upon DNA damage, EF-1 ε1 translocates to the nucleus where it interacts with ATM and ATR, resulting in p53 activation. In mice, loss of EF-1 ε1 results in high susceptibility to spontaneous tumors, strongly suggesting that EF-1 ε1 is a tumor suppressor.
Description: Rabbit polyclonal to AIMP3
Immunogen: KLH conjugated synthetic peptide derived from AIMP3
Specificity: ·Reacts with Human, Mouse, Pig, Dog and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 19 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
