Background: The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. α-protein kinase 3, also known as ALPK3, MAK or KIAA1330, is a 1,907 amino acid protein that contains one α-type protein kinase domain, one Ig-like (immunoglobulin-like) domain, and belongs to the protein kinase superfamily and ALPK subfamily. Members of the ALPK subfamily include ALPK1, ALPK2 and ALPK3, all of which recognize and phosphorylate specific sites that are surrounded by peptides that have an α-helical conformation. Specifically, α-protein kinase 1 targets Myosin I and is thought to play an important role in the apical trafficking of vesicles carrying raft-associated sucrase-isomaltase (SI).
Description: Rabbit polyclonal to ALPK3
Immunogen: KLH conjugated synthetic peptide derived from ALPK3
Specificity: ·Reacts with Human, Mouse, Pig, Dog and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 201 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
