Background: The high-mobility group (HMG) proteins 14 and 17 are abundant chromosomal proteins that bind to nucleosomes and enhance transcription. HMG-14 and HMG-17 also function as architectural elements, which alter the structure of the chromatin fiber and enhance transcription from chromatin templates. HMG-14/17 proteins modify the nucleosomal organization of the 30 nm chromatin fiber and mediate the unfolding of the higher order chromatin structure thereby facilitating access to the underlying DNA sequence. The nucleosomal binding protein 1 (NSBP1) is highly homologous to the HMG-14 and HMG-17 proteins and plays a pivotal role in chromatin remodeling. The human NSBP1 gene produces three mRNA transcripts with alternate polyadenylated sites, which are thought to mediate the stability of the mRNA. In androgen-independent prostate cancer cells, NSBP1 promotes cell growth and viability, which subsequently, makes NSBP1 a potential target for therapeutic purposes.
Description: Rabbit polyclonal to NSBP1
Immunogen: KLH conjugated synthetic peptide derived from NSBP1
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 32 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
