Background: The CSL-type zinc finger-containing proteins (ZCSLs) are homologs of the S. cerevisiae diphthamide methyltransferase proteins (DPHs). These enzymes are involved in the synthesis of diphthamide, a protein found on translation elongation factor EF-2 that is the target of bacterial ADP-ribosylating toxins. ZCSL1, ZCSL2 and ZCSL3 (CSL-type zinc finger-containing protein 1, 2 and 3, respectively) are members of the ZCSL family of proteins and each contain one DPH-type zinc finger. ZCSL2, also known as DPH3 or DESR1, is highly expressed in spleen, heart, lung, liver and thymus and is essential in the first step of dipthamide synthesis. Down-regulation of ZCSL2 increases the release of proteoglycans, suggesting a possible role in protein secretion. ZCSL1 and ZCSL3, also known as DPH3B and DPH4, respectively, are additional members of the ZCSL family of dipthamide-synthesizing enzymes.
Description: Rabbit polyclonal to DPH3
Immunogen: KLH conjugated synthetic peptide derived from DPH3
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 9 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/100-500;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
