Background: Lysine acetylation occurs in core histones, transcription factors and other proteins. This reversible modification is under the influence of signal-dependent association of substrates with acetyltransferases and deacetylases. Lysine acetylation generates specific docking sites for bromodomain proteins. Bromodomains of GCN5, PCAF, TAF1 and CBP are able to recognize acetyl-lysine residues in histones, HIV TAT, p53, c-Myb or MyoD. Trichostatin A (TSA), a histone deacetylase inhibitor, strongly increases acetylation of the N-terminal tails of Histone H3. Ethanol increases acetylation of Histone H3 at Lys 9 in a dose-dependent manner.
Description: Rabbit polyclonal to Acetyl Lysine
Immunogen: KLH conjugated synthetic peptide derived from Acetyl Lysine
Specificity: ·Reacts with Human, Mouse, Rabbit and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 15 kDa;
·Immunohistochemistry (Frozen/paraffin tissue section): 1/50-200;
·Immunocytochemistry: 1/100;
·Optimal working dilutions must be determined by the end user.
