Background: Caspase-3 (also designated CPP32; apopain; ICE-3; LICE; YAMA; Casp-3) Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a216-Asp-|-Gly-217 bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (By similarity). Subunit is compose of heterodimer of a 17 kDa (p17) and a 12 kDa (p12) subunit. Subcellular location at cytoplasmic. Ia belongs to the peptidase C14 family.
Description: Rabbit polyclonal to Caspase-3
Immunogen: KLH conjugated synthetic peptide derived from Caspase-3
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 32 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
