Background: CHIC2 (Cysteine-rich hydrophobic domain 2 protein), also known as BTL (BrX-like translocated in leukemia) and BTL/ETV6 fusion gene, is a 165 amino acid membrane protein whose gene is affected in a chromosomal translocation t(4;12)(q11;p13) occurring in acute myeloid leukemias (AML). CHIC2 is associated with the plasma membrane and vesicular structures, suggesting that it plays a role in regulating exocytosis. The cysteine-rich hydrophobic motif of CHIC2 contains cysteines that are palmitoylated, which is required for membrane association. In AML, the CHIC2 gene recombines with the TEL gene, resulting in a fusion protein containing the complete helix-loop-helix (HLH) and ETS DNA binding domains of TEL, but is transcribed via the CHIC2 promoter. Frequently, in systemic mast cell disease with associated eosinophilia, the gene encoding CHIC2 is deleted and a FIP1L1-PDGFR-α rearrangement is observed, a gene fusion which results in a constitutively active PDGFR-α.
Description: Rabbit polyclonal to CHIC2
Immunogen: KLH conjugated synthetic peptide derived from CHIC2
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 19 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
