Background: Centrosomes are the major microtubule-organizing centers of mammalian cells. They are composed of a centriole pair and surrounding microtubule-nucleating material termed pericentriolar material (PCM). Bipolar mitotic spindle assembly relies on two intertwined processes: centriole duplication and centrosome maturation. Failure to properly orchestrate centrosome duplication and maturation is subsequently linked to spindle defects, which can result in aneuploidy and promote cancer progression. CEP27 (Centrosomal protein of 27 kDa), also known as HAUS2 (HAUS augmin-like complex subunit 2), is a 235 amino acid protein that localizes to interphase centrosomes and mitotic spindle microtubules. As one of eight components of the HAUS augmin-like complex, CEP27 also contributes to regulation of mitotis spindle assembly and centrosomal integrity. There are two isoforms of CEP27 that are produced as a result of alternative splicing events.
Description: Rabbit polyclonal to CEP27
Immunogen: KLH conjugated synthetic peptide derived from CEP27
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 27 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
