Background: The immunophilins are a highly conserved family of cis-trans peptidyl-prolyl isomerases that bind to and mediate the effects of immunosuppressive drugs, such as cyclosporin, FK506 and rapamycin. FKBPL (FK506-binding protein-like), also known as WAF-1/CIP1 stabilizing protein 39 and DIR1, is a 349 amino acid immunophilin protein that recruits Hsp90 to stabilize p21 by preventing its proteosomal degradation. After ionizing radiation, FKBPL is transiently repressed and may play a role in induced radioresistance through a mechanism that increases rate of DNA repair in X ray exposed cells. siRNA knockdown of FKBPL results in decreased levels of cell cycle inhibitor p21WAF1 and increased ER-α phosphorylation in response to 17β-estradiol and tamoxifen. This evidence suggests that FKBPL may have an impact on tumor proliferative capacity and sensitivity to endocrine therapies.
Description: Rabbit polyclonal to WISP39
Immunogen: KLH conjugated synthetic peptide derived from WISP39
Specificity: ·Reacts with Human, Mouse, Pig, Dog and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 38 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
