Background: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA by transiently breaking and rejoining DNA strands. Although DNA topoisomerase I and DNA topoisomerase II relax both positive and negative supercoils, DNA topoisomerase III relaxes only negative supercoils. DNA topoisomerase III α exists as a long and a short isoform, which are produced by alternative splicing. DNA topoisomerase III α, which localizes to the nucleolus, is constitutively expressed and remains at high levels throughout the cell cycle in HL-60 cells. DNA topoisomerase III β exists as three isoforms, namely β1, β2, and β3, also produced by alternative splicing. DNA topoisomerase III β1 is expressed in testes, heart, and skeletal muscle, whereas β2 is expressed in thymus, kidney, and pancreas.
Description: Rabbit polyclonal to Topoisomerase III
Immunogen: KLH conjugated synthetic peptide derived from Topoisomerase III
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 112 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
