Background: Hic-5 contains four LIM motifs and seven zinc finger domains. In the cell, Hic-5 localizes to the nuclear matrix and focal adhesion complexes where the LIM domains mediate the interactions of Hic-5 with focal adhesions. Known also as transforming factor beta 1 induced transcript 1, Hic-5 shares extensive homology with the structural protein paxillin, which is involved in the regulation of focal adhesion dynamics. Hic-5 inhibits integrin-mediated cell spreading on Fibronectin by out competing paxillin for focal adhesion kinase and thereby preventing downstream signal transduction. Increased expression of Hic-5 leads to cellular senescence in developing fibroblasts. During myogenesis, expression of Hic-5 blocks differentiation and induces apoptosis of developing myoblasts. The gene encoding human Hic-5 maps to chromosome 16.
Description: Rabbit polyclonal to HIC5
Immunogen: KLH conjugated synthetic peptide derived from HIC5
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 48 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
