Background: In the central nervous system (CNS), glycine-mediated inhibitory neurotransmission is essential to voluntary motor control and reflex responses. Glycine binds to glycine receptors (GlyR) in the post synaptic neuronal membranes. GlyR, Gamma-aminobutryic acid, serotonin and acetylcholine comprise an evolutionally conserved superfamily of ligand-gated ion channels. The pentameric subunit structure of GlyR consists of two types of glycosylated membrane proteins, alpha 1 through alpha 4 and Beta, and an associated peripheral membrane protein, which combine to form a chloride-selective ion channel. In humans, the composition of the pentamer changes from alpha 2 subunits in the fetal CNS to alpha 1 and Beta subunits in the adult CNS. Fast potentiation of GlyR by intracellular Ca2+ in the brainstem and midbrain indicate an important role for Ca2+ in modulation glycinergic synapses. The genes encoding human GlyR alpha 1, alpha 2, alpha 3 and Beta subunits map to chromosomes 5q33.1, Xp22.2, 4q34.1 and 4q32.1, respectively.
Description: Rabbit polyclonal to GLRA3
Immunogen: KLH conjugated synthetic peptide derived from GLRA3
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 54 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
