Background: GLIS1 is a 789 amino acid protein encoded by the mouse gene Glis1. Located in the nucleus, GLIS1 acts as both a repressor and activator of transcription. GLIS1 belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. GLIS1 is expressed in a temporal and spatial manner during development, with expression being most prominent in several defined structures of mesodermal lineage, including craniofacial regions, branchial arches, somites, vibrissal and hair follicles, limb buds, and myotomes. GLIS1 is a a novel Krüppel-like protein that binds to the consensus sequence 5'-GACCACCCAC-3'. The Kruppel gene family characterized by a consensus C2-H2 zinc finger domain and is believed to function as a transcription activator in the vertebrate Sonic hedgehog (Shh)-patched signal transduction pathway. Understanding GLI gene regulation may be of importance to understanding causes of human birth defects and cancer.
Description: Rabbit polyclonal to GLIS1
Immunogen: KLH conjugated synthetic peptide derived from GLIS1
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 65 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
