Background: Eukaryotic protein phosphorylation and dephosphorylation on serine and threonine residues regulates numerous cell functions, including division, homeostasis and apoptosis. A group of proteins that play a major role in this process are the serine/threonine protein phosphatases. Protein phosphatase (PP) holoenzyme is a trimeric complex that contains a regulatory subunit, a variable subunit and a catalytic subunit. PP2C family members are negative regulators of cell stress response pathways. The PP2C∫ enzyme has broad specificity and is highly expressed in the heart and skeletal muscle. It may be involved in cell cycle control as it dephosphorylates the cyclin-dependent kinases (CDKs), CDK2 and CDK6, in vitro. Overexpression of PP2C∫ can cause cell-growth arrest or cell death.
Description: Rabbit polyclonal to PPM1B
Immunogen: KLH conjugated synthetic peptide derived from PPM1B
Specificity: ·Reacts with Human, Mouse, Dog and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 53 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
